Part:BBa_K1073018
Autoinducer synthetase LasI ( producing N-3-oxododecanoyl-HSL) with RBS and double terminator
The LasI protein which produces the chemical autoinducer signal N-3-oxododecanoyl-HSL was combined with a RBS and a double terminator in order to facilitate the construction of an expression cassette containing LasI.
Usage and Biology
In combination with a promoter of your choice this device can be used to create a LasI expression cassette. The RBS used will tune a medium expression level. Rate of transcription can be tuned by strength of promoter. The double terminator will prevent further transcription downstream of lasI.
The N-3-oxododecanoyl-HSL autoinducer molecules produced by LasI bind to LasR. This complex will bind to specific sequences in the regulatory region of the las promoter and induce gene expression.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 680
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 242
- 1000COMPATIBLE WITH RFC[1000]
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